Scott Plafker, Ph.D., R.Ph.

Education

Postdoctoral Training 1998-2003, University of Virginia School of Medicine

PhD 1998, Johns Hopkins University School of Medicine

BS Pharmacy 1992, Rutgers University College of Pharmacy

Research Review

The guiding principle of our research program is that diet and nutrition are the foundation of health and healthy aging. This means that food choices not only play a critical role in preventing and treating diseases but can also be an underlying cause of some ailments. We are particularly interested in understanding how diet contributes to diseases of the eye and how nutrition can be leveraged to prevent and treat vision loss. Our research focuses on two diseases that cause blindness: (1) age-related macular degeneration (AMD) and (2) the optic neuritis associated with multiple sclerosis (MS). Both of these diseases can culminate in irreversible blindness and dramatically reduce the quality of life for patients.

We have two major projects in the lab currently. One aims to identify the beneficial effects of sulforaphane, a compound found in green, leafy vegetables like broccoli, brussel sprouts and kale. Our studies are focused on determining the impact of sulforaphane on rejuvenating mitochondria, the energy producing organelles in our cells that decrease in function with aging and are widely believed to play a causal role in AMD onset and progression. A second project focuses on comparing the impact of high versus low carbohydrate diets on the motor and visual symptoms of MS. This work is being done using a mouse model that mimics many of the cardinal symptoms experienced by MS patients. It is our ultimate goal to empower patients with dietary information and practices that they can apply each day to improve and maintain their overall health and vision.

Plafker Lab

Scott Plafker, Ph.D., R.Ph.

Principal Investigator

Kendra S. Plafker

Lab Manager

Jocelyn (Josie) Roberts

Senior Research Technician

Katarzyna (Kasia) Zyla, MS

Graduate Student

Contact

Oklahoma Medical Research Foundation

Chapman Building

Second Floor, Room MC205

Oklahoma City OK 73104

Phone: (405) 271-1735

plafkers@omrf.org

Publications

CV Link

Website

Our research program can be summarized by the adage, “Food as medicine.” We believe that food and nutrition reside at the center of health and healthy aging. Of course, there are two sides to every coin, and our food choices can promote health or disease. Therefore, we seek to explain at the biochemical, cell biological, and metabolic levels how certain food-derived compounds and diets promote health whereas others drive disease. In this context, our laboratory focuses on how cells adapt to stresses and how nutrition impacts these responses. A central theme guiding our work is that healthy aging requires cells to retain the capacity to sense stresses, and then respond and adapt in a measured way to restore homeostasis. Examples of the stresses we study include oxidative stress, starvation, and hyperglycemia. The failure to appropriately deal with such stresses results in maladaptive responses that manifest as inflammation, tissue damage, and senescence. We are particularly interested in understanding how the transcription factors Nrf2, HIF-1a, and p53 function in a coordinated fashion to neutralize stresses, restore homeostasis, and prevent senescence. Loss or gain of function of these anti-stress transcription factors is linked to diseases ranging from cancers to age-related neurodegeneration to autoimmune disorders.

A main project in the laboratory involves testing how diets with either high or low amounts of carbohydrates impact the motor and vision deficits observed in multiple sclerosis (MS). We are using a mouse model of MS that reconstitutes many of the pathologies experienced by patients and our ultimate goal is to develop nutritional intervention strategies for MS patients. At the mechanistic level, we are exploring how anti-stress transcription factors respond to excess dietary carbohydrates and how various populations of T effector cells expand or contract as a function of diet. We anticipate that carbohydrate restriction will mitigate disease symptoms by reducing pathological T cells and expanding immune tolerant T regulatory cells.

A second project in our laboratory investigates the therapeutic potential and limitations of sulforaphane, a natural compound derived from cruciferous vegetables (e.g., broccoli, Brussels sprouts, kale). Sulforaphane is a potent activator of the Nrf2 transcription factor and is currently being tested in clinical trials for many diseases. In 2017, we discovered that sulforaphane can alter mitochondrial and peroxisomal morphology. Our current focus is to determine the consequences of these morphological changes on organelle function and cellular homeostasis. We are also testing whether sulforaphane can preserve vision in mouse models designed to mimic features of age-related macular degeneration (AMD), a leading cause of irreversible vision loss in people over the age of 65. AMD is typically characterized by death of the light-sensing photoreceptors in the posterior eye, and we are examining which protective properties of sulforaphane require Nrf2 and whether diets enriched in sulforaphane can preserve vision in young and old mice.